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Cells on Hierarchically-Structured Platforms Hosting Functionalized Nanoparticles
Biomaterials Science 2018, 6, 1469–1479 DOI: 10.1039/C7BM00904F
Abstract
In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogeneously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e., oligopeptides, small molecules, and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on the hierarchical substrates incorporating AuNPs carrying the RGD peptide showed impressive growth and NGF-induced differentiation of the PC12 cells compared to that on the NPs-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.
For citation:
Simitzi, C.; Harimech, P. K.; Spanou, S.; Lanara, C.; Heuer-Jungemann, A.; Manousaki, A.; Fotakis, C.; Ranella, A.; Kanaras, A. G.; Stratakis, E.
"Cells on Hierarchically-Structured Platforms Hosting Functionalized Nanoparticles"
Biomater. Sci. 2018, 6, 1469–1479. DOI: 10.1039/C7BM00904F