Enzyme-dependent assembly of DNA/gold nanostructures is achieved using DNA-capped gold colloids and DNA processing enzymes. Particle-bound DNA double strands are cleaved at designated sites by a restriction endonuclease (see picture). In a subsequent step, the particles are linked to each other through these activated sites by a DNA ligase. This new strategy enables the multistep synthesis of nanostructures.
For citations:
Kanaras A. G.; Wang Z.; Bates A. D.; Cosstick R.; Brust M.
Towards multistep nanostructure synthesis: programmed enzymatic self-assembly of DNA/gold systems.
Angew. Chem. Int. Ed., 2003, 42 (2), 191-194
DOI: 10.1002/anie.200390075,
Angew. Chem. 2003, 115 (2), 201-204
DOI: 10.1002/ange.200390043