Publications
Table of Contents (TOC)
Preparation of Peptide Functionalized Gold Nanoparticles Using One Pot EDC/sulfo-NHS Coupling
Langmuir, 2011, 27 (16), 10119-10123
Abstract
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e., in amino acid coupling), their use in the coupling of peptides to water-soluble carboxylic-terminated colloidal gold nanoparticles remains challenging. In this paper, we present an optimization study for the successful coupling of the KPQPRPLS peptide to spherical and rod-like colloidal gold nanoparticles. We show that the concentration, reaction time, and the chemical environment are all critical to achieve the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
For citations:
Bartczak, D.; Kanaras* A. G.
Preparation of Peptide Functionalized Gold Nanoparticles Using One Pot EDC/sulfo-NHS Coupling
Langmuir, 2011, 27 (16), 10119-10123
DOI: 10.1021/la2022177