Selected Publications

2014

Krypotou E, Diallinas G. 2014. Transport assays in filamentous fungi: Kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genet Biol 63:1-8.

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2013

Karachaliou M, Amillis S, Evangelinos M, Kokotos AC, Yalelis V, Diallinas G. 2013. The arrestin-like protein ArtA is essential for ubiquitination and endocytosis of the UapA transporter in response to both broad-range and specific signals. Mol Microbiol 88:301-17.

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Diallinas G. 2013.  Allopurinol and xanthine use different translocation mechanisms and trajectories in the fungal UapA transporter. Biochimie 95:1755-64

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2012

Leung J, Cameron AD, Diallinas G, Byrne B. 2012. Stabilizing the heterologously expressed uric acid-xanthine transporter UapA from the lower eukaryote Aspergillus nidulans. Mol Membr Biol 30:32-42.

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Kosti V, Lamprinidis G, Myrianthopoulos V, Diallinas G, Mikros E. 2012. Identification of a substrate recognition and transport pathway in a Eukaryotic Member of the Nucleobase-Ascorbate Transporter (NAT) Family. PLoS One 7:e41939.

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Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. 2012. Modeling, Substrate Docking and Mutational Analysis Identify Residues essential for the function and specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter.  J Biol Chem 287:36792-803.

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2011

Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Diallinas G. 2011. A substrate translocation trajectory in a cytoplasm-facing topological model of the monocarboxylate/H symporter Jen1p. Mol Microbiol 81:805-17.

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Amillis S, Kosti V, Pantazopoulou A, Mikros E, Diallinas G. 2011. Mutational analysis and modeling reveal functionally critical residues in transmembrane segments 1 and 3 of the UapA transporter. J Mol Biol 411:567-80.

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2010

Gournas C, Amillis S, Vlanti A and Diallinas G. 2010. Transport-dependent endocytosis and turnover of a uric acid-xanthine permease. Mol Microbiol 75:246-260.

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Conde A, Diallinas G, Chaumont F, Chaves M, Gerós H. 2010. Transporters, channels, or simple diffusion? Dogmas, atypical roles and complexity in transport systems. Int J Biochem Cell Biol 42:857-68.

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Kosti V, Papageorgiou I and Diallinas G. 2010. Dynamic elements at both cytoplasmic- and extracellular-facing sides of the UapA transporter selectively control the accessibility of substrates to their translocation pathway. J Mol Biol 397:1132-43.

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2009

Lemuh ND, Diallinas G, Frillingos S, Mermelekas G, Karagouni AD and  Hatzinikolaou DG. 2009. Purification and partial characterization of the xanthine-uric acid transporter (UapA) of Aspergillus nidulans. Protein Expr Purif 63:33-39.

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Hamari Z, Amillis S, Drevet C, Apostolaki A, Vágvölgyi C, Diallinas G, Scazzocchio C. 2009. Convergent evolution and orphan genes in the Fur4p-like family and characterisation of a general nucleoside transporter in Aspergillus nidulans. Mol Microbiol. 73:43-57.

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Vangelatos I, Vlachakis D, Sophianopoulou V, Diallinas G. 2009. Modelling and mutational evidence identify the substrate binding site and functional elements in APC amino acid transporters. Mol Membr Biol 26:356-370.

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2008

Goudela S, Reichard U, Amillis S and Diallinas G. 2008. Characterization and kinetics of the major purine transporters in Aspergillus fumigatus. Fungal Genet Biol 45:459-72.

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Vlanti A and Diallinas G. 2008. The Aspergillus nidulans FcyB cytosine-purine scavenger is highly expressed during germination and in reproductive compartments and is down-regulated by endocytosis. Mol Microbiol 68: 959-977.

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Gournas C, Papageorgiou I and Diallinas G. 2008. The Nucleobase-Ascorbate Transporter (NAT) family: Genomics, evolution, structure-function relationships and physiological role.  Mol BioSys 4: 404 -416.

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Papageorgiou I, Gournas C, Vlanti A, Amillis S, Pantazopoulou A  and Diallinas G. 2008. Specific interdomain synergy in the UapA transporter determines its unique specificity for uric acid among NAT carriers. J Mol Biol 382:1121-1135.

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Diallinas G. 2008. Biochemistry. An almost-complete movie. Science 12:1644-5.

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Diallinas G and Gournas C. 2008. Structure-function relationships in the Nucleobase-Ascorbate Transporter (NAT) family: Lessons from model microbial genetic systems. Channels, 2: 363-372.

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Issue cover

2007

Pantazopoulou A, Lemuh ND, Hatzinikolaou DG, Drevet C, Cecchetto G, Scazzocchio C and Diallinas G. 2007. Differential physiological and developmental expression of the UapA and AzgA purine transporters in Aspergillus nidulans. Fungal Genet Biol 44: 627-640.

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Issue cover

   

Amillis S, Hamari Z, Roumelioti K, Scazzocchio C and Diallinas G. 2007.  Regulation of Expression and Kinetic Modeling of Substrate Interactions of a Uracil Transporter in Aspergillus nidulans. Mol Membr Biol 24:206-214.

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Pantazopoulou A and Diallinas G, 2007. Fungal Nucleobase transporters. FEMS Microbiol Rev 31: 657-675.

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Soares-Silva I,  Paiva S,  Diallinas G, and Casal M. 2007. The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H+ symporter subfamily defines the function of the substrate translocation pathway. Mol Membr Biol 24: 464-474.

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2006

Vlanti A, Amillis S, Koukaki M, and Diallinas G. 2006. The last transmembrane segment of UapA, a member of the nucleobase-ascorbate transporter (NAT) family, includes a substrate-selectivity filter and is necessary for ER-exit. J Mol Biol 357: 808-819.

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Goudela S, Tsilivi H and Diallinas G. 2006. Comparative kinetic analysis of AzgA and Fcy21p, prototypes of the two major fungal hypoxanthine-adenine-guanine transporter families. Mol Membr Biol 23: 291-303.

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Pantazopoulou A and Diallinas G. 2006. The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Mol Membr Biol 23: 337-248.

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2005

S. Goudela, Karatza P, Koukaki M, Frillingos S and Diallinas G. 2005. Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Mol Membr Biol 22: 263-275.

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Koukaki M, Vlanti A, Goudela S, Pantazopoulou A, Gioule H, Tournaviti S and Diallinas G. 2005. The Nucleobase-Ascorbate Transporter (NAT) Signature Motif in UapA Defines the Function of the Purine Translocation Pathway. J Mol Biol 350: 499-513.

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2004

Cecchetto G, Amillis S, Diallinas G, Scazzocchio C, Drevet C, 2004. The AzgA purine transporter of Aspergillus nidulans: characterisation of a protein belonging to a new phylogenetic cluster. J Biol Chem 279: 3132-3141.

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Amillis S, Cecchetto G, Sophianopoulou V, Koukaki M, Scazzocchio C and Diallinas G. 2004. Transcriptional activation of purine transporters during the conidial isotropic growth phase of Aspergillus nidulans. Mol Microbiol 52: 205-216.

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2003

Koukaki M, Giannoutsou E, Karagouni A, Diallinas G. 2003. A novel improved method for Aspergillus nidulans transformation. J Microbiol Methods 55: 687-695.

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2001

Argyrou E, Sophianopoulou V, Schultes N, and Diallinas G. 2001. Functional characterization of a maize purine transporter by expression in Aspergillus nidulans. Plant Cell 13: 953-964.

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Amillis S, Koukaki M and Diallinas G. 2001. Substitution F569S converts UapA, a specific uric acid-xanthine transporter, into a broad specificity transporter for purine-related solutes. J Mol Biol 313: 765-774.

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2000

De Koning H and Diallinas G. 2000. Nucleobase transporters. Mol Membr Biol 17: 75-94.

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Meintanis C, Karagouni A and Diallinas G. 2000. Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family. Mol Membr Biol 17:47-57.

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1998

Diallinas G, Valdez J, Sophianopoulou V, Rosa A and Scazzocchio C. 1998. Chimeric protein analysis reveals a region involved in function and specificity of purine transporters in the filamentous fungus Aspergillus nidulans conserved in bacteria, plants and metazoans. EMBO J 17: 3827-3837.

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